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バイオバンキングのための切除肺組織からの気管支上皮細胞の単離と高分化気液界面培養の樹立
JoVE Journal
Immunologia e infezioni
This content is Free Access.
JoVE Journal Immunologia e infezioni
Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
DOI:

08:42 min

May 26, 2023

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Capitoli

  • 00:04Introduction
  • 00:47Isolation of Bronchial Epithelial Cells from Human Lung Tissue
  • 02:17Cryopreservation of Human Primary Bronchial Epithelial Cells (PBECs)
  • 03:28Thawing Cryopreserved PBECs and Growing Them for Culture on Inserts
  • 04:23Establishing an Air‐Liquid Interface Culture with Primary Bronchial Epithelial Cells (ALI‐PBEC)
  • 06:03Results: Characterization of ALI Cultures Using Cell‐Specific Marker Genes
  • 07:41Conclusion

Summary

Traduzione automatica

ここでは、長期バイオバンキングのための初代気管支上皮細胞の単離と増殖、および気液界面での培養による分化上皮細胞の生成のための、再現性があり、手頃な価格で堅牢な方法を紹介します。

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