/

/

使用基于光学的系统对水凝胶嵌入的完整小鼠肌纤维进行高通量收缩测量

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

High-Throughput Contractile Measurements of Hydrogel-Embedded Intact Mouse Muscle Fibers Using an Optics-Based System

Please note that all translations are automatically generated. Click here for the English version.

使用基于光学的系统对水凝胶嵌入的完整小鼠肌纤维进行高通量收缩测量

DOI:

10.3791/65103-v

•

07:35 min

•

May 05, 2023

•

Leander A. Vonk*¹, Osman Esen*¹, Michaela Yuen⁴, Tyler J. Kirby^2,3

¹Department of Physiology,Amsterdam UMC, ²Amsterdam Cardiovascular Sciences, Heart Failures and Arrhythmias,Amsterdam UMC, ³Amsterdam Movement Sciences, Tissue Function and Regeneration,Amsterdam UMC, ⁴Discipline of Child and Adolescent Health, Faculty of Health and Medicine,University of Sydney

Capitoli

00:04Introduction
00:58Murine Flexor Digitorum Brevis Muscle Dissection
02:07Muscle Digestion, Trituration, and Gravity Sedimentation
02:59Fiber Embedding
03:44Optics‐Based Contractile Measurements
06:20Results: Embedded Muscle Contraction Assessment
06:59Conclusion

Summary

Traduzione automatica

骨骼肌功能可以通过量化孤立的肌肉纤维的收缩力来评估，传统上使用费力的低通量方法。在这里，我们描述了一种基于光学的高通量方法来量化水凝胶嵌入的肌肉纤维的收缩性。这种方法在药物筛选和治疗开发方面有应用。

Tags

Artificial Intelligence AI Writing Assistant AI-generated Content Copywriting Content Generation Content Creation Text Generation Natural Language Processing

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

对大鼠的过程<em在原位</em骨骼肌收缩性能

使用高通量形状的RNA二级结构预测

高通量清醒小鼠肾小球滤过率的测定方法

钙火花的评估在正常肌肉纤维

高通量筛选转录后调控基因的化学调节剂

激酶底物对鉴定使用高通量筛选

从小鼠骨骼肌高通量微孔板呼吸测量最低数量的隔离线粒体

多酶筛选使用高通量基因酶的筛选系统

骨骼肌完整的线粒体的分离差速离心高分辨率测量呼吸运动

利用斑马鱼对肠道转运时间进行高通量测量

Read Article