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Evidence for EpCAM and Cytokeratin Expressing Epithelial Cells in Normal Human and Murine Blood and Bone Marrow
JoVE Journal
Biologia
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JoVE Journal Biologia
Evidence for EpCAM and Cytokeratin Expressing Epithelial Cells in Normal Human and Murine Blood and Bone Marrow

Evidence for EpCAM and Cytokeratin Expressing Epithelial Cells in Normal Human and Murine Blood and Bone Marrow

DOI:
10.3791/65118-v

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08:02 min

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April 21, 2023

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, ,
1The Hormel Institute,University of Minnesota

Capitoli

  • 00:04Introduction
  • 00:58Harvesting Bone Marrow from Mice
  • 03:54Red Blood Cell Lysis of Bone Marrow
  • 04:38Flow Cytometry
  • 06:18Results: Flow Cytometric Analysis of EpCAM+ Cells in Murine and Human Bone Marrow and Blood
  • 07:26Conclusion

Summary

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This paper presents a reproducible method with new findings on the presence of epithelial cells in normal human and mouse blood and bone marrow using flow cytometry and immunofluorescence microscopy. Krt1-14;mTmG transgenic mice were used as an in vivo method to confirm these findings.

Tags

Keywords: EpCAMCytokeratinEpithelial CellsBloodBone MarrowFlow CytometryCirculating Tumor CellsBiologia dello sviluppoStem Cell BiologyRegenerative TherapiesWound HealingMouseBone HarvestBone Marrow Extraction

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