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Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids
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JoVE Journal Bioingegneria
Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

Bioorthogonal Chemical Imaging of Cell Metabolism Regulated by Aromatic Amino Acids

DOI:
10.3791/65121-v

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10:42 min

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May 12, 2023

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, , , , ,
1Shu Chien-Gene Lay Department of Bioengineering,University of California San Diego

Capitoli

  • 00:05Introduction
  • 00:54Media Preparation
  • 02:10Cell Sample Preparation
  • 05:22Spontaneous Raman Spectroscopy Measurement
  • 06:15Imaging Experiments with Two-Photon Excitation Fluorescence and Stimulated Raman Scattering
  • 08:25Results: Visualizing Metabolic Dynamics and 3D Lipid Droplet Distribution in HeLa Cells
  • 09:48Conclusion

Summary

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We present a protocol to directly visualize metabolic activities in cells regulated by amino acids using deuterium-oxide (heavy water D2O) probed stimulated Raman scattering (DO-SRS) microscopy, which is integrated with two-photon excitation fluorescence microscopy (2PEF).

Tags

BioorthogonalChemical ImagingCell MetabolismAromatic Amino AcidsRaman ImagingTwo-photon FluorescenceSecond Harmonic GenerationDMEMDeuterium OxidePhenylalanineTryptophanMethionineThreoninePoly-D-LysineLamininTrypsin

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