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Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

JoVE Journal
Biochimica
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JoVE Journal Biochimica

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies

DOI:

10.3791/65123-v

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10:23 min

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May 19, 2023

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Juliane Corrêa Glória^2,3, Yury Oliveira Chaves^3,4, Adélia Marques de Figueiredo, Caio Coutinho de Souza, Eliza Raquel Duarte da Silva, Jeniffer Clorives Lopes Batista⁵, Paulo Afonso Nogueira^3,5, Luis André Morais Mariúba^2,3,5

¹Programa de Pós-Graduação em Biotecnologia da Universidade Federal do Amazonas – UFAM, ²Instituto Leônidas e Maria Deane (ILMD),Fundação Oswaldo Cruz (FIOCRUZ), ³Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro do Instituto Leônidas e Maria Deane (ILMD),Fundação Oswaldo Cruz (FIOCRUZ), ⁴Programa de Pós-graduação em Ciências Aplicadas à Hematologia – Universidade Estadual do Amazonas/Fundação Hospitalar de Hematologia e Hemoterapia do Amazonas, ⁵Programa de Pós-Graduação em Imunologia Básica e Aplicada, Instituto de Ciências Biológicas,Universidade Federal do Amazonas (UFAM)

Capitoli

00:04Introduction
00:45Extraction of IgY from Egg Yolks
02:27Saturation Curve of IgY Antibodies to Latex Beads
04:02Flow Cytometry Assay Based on Latex Beads for Antigen Detection
05:05Flow Cytometer Reading of the Samples and Sample Analysis
07:33Results: Electrophoretic Profile of the IgY‐PfHRP2 Antibody
09:29Conclusion

Summary

Traduzione automatica

This protocol describes a methodology for the preparation of latex beads for assays using IgY antibodies for antigen detection.

Tags

Keywords: Cytometric Bead Assay Egg-yolk Antibodies IgY Antibodies Flow Cytometry Immunoassay Latex Beads PfHRP2 Standardization Bradford Assay EDC NHS

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