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High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements
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1Department of Physics,Pohang University of Science and Technology (POSTECH), 2School of Biological Sciences,Seoul National University, 3Institute for Molecular Biology and Genetics,Seoul National University, 4School of Interdisciplinary Bioscience and Bioengineering,Pohang University of Science and Technology (POSTECH)
Capitoli
- 00:04Introduction
- 00:59Apparatus Construction
- 02:36Flow Cell Fabrication
- 03:50Assembly of Bead‐Tether Constructs
- 04:20Identification of Target Constructs
- 05:00Calibration of Magnetic Force
- 05:50Bead Tracking for Extension Measurement
- 06:28Results: Effect of Magnetic Force on DNA Constructs
- 08:11Conclusion
Here, we describe a high-speed magnetic tweezer setup that performs nanomechanical measurements on force-sensitive biomolecules at the maximum rate of 1.2 kHz. We introduce its application to DNA hairpins and SNARE complexes as model systems, but it will be also applicable to other molecules involved in mechanobiological events.
Tags
Magnetic TweezersNanomechanical MeasurementsForce-sensitive ElementsBiomoleculesStructural ChangesNucleic AcidsProteinsMechanosensitive ProteinsCardiovascular DisordersMusculoskeletal DisordersHigh-speed CMOS CameraMotorized Linear StageRotary Stepper MotorSuper Luminescent DiodePiezo Lens ScannerCoverslip PreparationPegylation
