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Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice
JoVE Journal
Biologia
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JoVE Journal Biologia
Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice
DOI:

04:10 min

March 31, 2023

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Capitoli

  • 00:04Introduction
  • 00:29Preparation of Intubation Platform and Test Compound
  • 01:39Non‐Invasive Oropharyngeal Instillation
  • 02:56Results: Effect of LPS Tracheal Instillation on Acute Lung Injury
  • 03:38Conclusion

Summary

Traduzione automatica

Here, we propose a protocol for intratracheal lipopolysaccharide (LPS) delivery via noninvasive oropharyngeal endotracheal intubation. This method minimizes the trauma of the surgical procedure for the animal and accurately delivers LPS to the trachea and then to the lungs.

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