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Noninvasiv intratrakeal lipopolysaccharidinstillation hos mus
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Biologia
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JoVE Journal Biologia
Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice

Noninvasiv intratrakeal lipopolysaccharidinstillation hos mus

DOI:
10.3791/65151-v

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04:10 min

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March 31, 2023

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, , , , , , , ,
1State Key Laboratory of Southwestern Chinese Medicine Resources, College of Pharmacy, Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, 2Department of Pharmacy,The Second Affiliated Hospital of Hainan Medical University, 3The School of Preclinical Medicine,Chengdu University

Capitoli

  • 00:04Introduction
  • 00:29Preparation of Intubation Platform and Test Compound
  • 01:39Non‐Invasive Oropharyngeal Instillation
  • 02:56Results: Effect of LPS Tracheal Instillation on Acute Lung Injury
  • 03:38Conclusion

Summary

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Her foreslår vi en protokol for intratracheal lipopolysaccharid (LPS) levering via ikke-invasiv orofaryngeal endotracheal intubation. Denne metode minimerer traumet ved den kirurgiske procedure for dyret og leverer nøjagtigt LPS til luftrøret og derefter til lungerne.

Tags

Keywords: NoninvasiveIntratrachealLipopolysaccharideLPSInstillationMiceTracheaIntubationCannulaPentobarbitalTNF-alphaLung Dry-wet Weight Ratio

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