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Ikke-invasiv intratrakeal lipopolysakkarid instillasjon hos mus
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Biologia
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JoVE Journal Biologia
Noninvasive Intratracheal Lipopolysaccharide Instillation in Mice

Ikke-invasiv intratrakeal lipopolysakkarid instillasjon hos mus

DOI:
10.3791/65151-v

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04:10 min

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March 31, 2023

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, , , , , , , ,
1State Key Laboratory of Southwestern Chinese Medicine Resources, College of Pharmacy, Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, 2Department of Pharmacy,The Second Affiliated Hospital of Hainan Medical University, 3The School of Preclinical Medicine,Chengdu University

Capitoli

  • 00:04Introduction
  • 00:29Preparation of Intubation Platform and Test Compound
  • 01:39Non‐Invasive Oropharyngeal Instillation
  • 02:56Results: Effect of LPS Tracheal Instillation on Acute Lung Injury
  • 03:38Conclusion

Summary

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Traduzione automatica

Her foreslår vi en protokoll for intratrakeal lipopolysakkarid (LPS) levering via ikke-invasiv orofaryngeal endotrakeal intubasjon. Denne metoden minimerer traumer av den kirurgiske prosedyren for dyret og leverer nøyaktig LPS til luftrøret og deretter til lungene.

Tags

Keywords: NoninvasiveIntratrachealLipopolysaccharideLPSInstillationMiceTracheaIntubationCannulaPentobarbitalTNF-alphaLung Dry-wet Weight Ratio

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