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Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

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多重免疫荧光结合空间图像分析，用于肿瘤微环境的临床和生物学评估

JoVE Journal
Ricerca sul cancro

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JoVE Journal Ricerca sul cancro

Multiplex Immunofluorescence Combined with Spatial Image Analysis for the Clinical and Biological Assessment of the Tumor Microenvironment

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多重免疫荧光结合空间图像分析，用于肿瘤微环境的临床和生物学评估

Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

DOI:

10.3791/65220-v

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01:36 min

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June 02, 2023

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Nicolas Huyghe, Elena Benidovskaya, Simon Beyaert, Aurélie Daumerie, Finoula Maestre Osorio, Frank Aboubakar Nana³, Caroline Bouzin, Marc Van den Eynde⁵

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

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Keywords: Multiplex Immunofluorescence Spatial Image Analysis Tumor Microenvironment Colorectal Cancer Metastatic Disease Immunotherapy Biomarkers RNA Sequencing Whole Exome Sequencing TCR Sequencing Circulating Cell-free DNA Analysis Fluorescence In Situ Hybridization Tyramide Signal Amplification Image Analysis Spatial Analysis Tumor Compartments Cell-cell Interactions

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