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1Department of Molecular Biosciences,The University of Texas at Austin, 2BEACON Center for the Study of Evolution in Action,Michigan State University, 3Department of Microbiology and Molecular Genetics,Michigan State University, 4Department of Integrative Biology,Michigan State University, 5Biological Sciences Program,Michigan State University
Capitoli
- 00:03Introduction
- 02:02Daily Transfers of LTEE Populations
- 06:04Competitive Fitness Assays
- 06:44Prepare Supplies
- 08:24Day -2: Revive Competitors
- 09:09Day -1: Precondition Competitors
- 09:47Day 0: Begin Competition
- 11:16Alternative:Continue Three-Day Competition
- 11:43Day 1 or 3: Finish Competition
- 12:41Calculate and Plot Fitness
- 12:52Representative Results Appearance and turbidity of LTEE Cultures
- 13:22Representative Results Co-culture Competition Results
- 14:39Conclusion
Denne protokol beskriver, hvordan man opretholder Escherichia coli Long-Term Evolution Experiment (LTEE) ved at udføre sine daglige overførsler og periodiske frysninger, og hvordan man udfører konkurrenceanalyser for at måle fitnessforbedringer i udviklede bakterier. Disse procedurer kan tjene som en skabelon for forskere, der starter deres egne mikrobielle evolutionseksperimenter.
