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Dot-Blot Assay for Detection of Leptospira DNA in Water Samples
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聚合酶链反应和斑点-印迹杂交用于水样中 钩端螺旋体 检测
JoVE Journal
Biologia
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JoVE Journal Biologia
Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples
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聚合酶链反应和斑点-印迹杂交用于水样中 钩端螺旋体 检测

Dot-Blot Assay for Detection of Leptospira DNA in Water Samples

DOI:
10.3791/65435-v

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00:32 min

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June 14, 2024

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, , , , ,
1Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, 2Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, 3Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Tags

Keywords: Polymerase Chain ReactionDot-Blot HybridizationLeptospiraWater SamplesDNA DetectionNon-Isotopic TechniqueProbe HybridizationNylon MembraneAnti-Digoxigenin AntibodyPCR-Dot-Blot AssayWater ConcentrationEcosystem Analysis

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