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Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model
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Neuroscienze
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JoVE Journal Neuroscienze
Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

DOI:
10.3791/65448-v

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07:37 min

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January 26, 2024

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, , , , , , ,
1Wilmer Eye Institute,Johns Hopkins University School of Medicine, 2Department of Genetic Medicine,Johns Hopkins University School of Medicine

Capitoli

  • 00:00Introduction
  • 00:31Animals
  • 00:58Collect Neural Retina
  • 01:58Prepare Donor Retinal Suspension
  • 02:19Prepare Donor Retinal Sheets
  • 02:36Prepare Mouse Recipients
  • 03:46Subretinal Transplantation of Retinal Grafts
  • 05:12Post-surgery Administration
  • 05:48Risultati
  • 07:04Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol presents a transpupillary vision-guided trans-scleral approach to safely and precisely deliver subretinal cellular grafts, with a low rate of surgical complications, in mouse recipients with or without retinal degeneration.

Tags

Transpupillary-guidedTrans-scleralSubretinal GraftsRetinal DegenerationMouse ModelEGFPOPN1LW-EGFP/NRLRd1/NSPapainRetinal SheetAnesthesiaPupil DilationTranspupillary Visualization

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