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Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes
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Genetica
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JoVE Journal Genetica
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

DOI:
10.3791/65472-v

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05:34 min

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July 14, 2023

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, , ,
1Unità di Genetica, Dipartimento di Biologia,University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology,Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico

Capitoli

  • 00:15Introduction
  • 00:45Preparation of Samples and Mutagenic Treatment
  • 01:19Fixation
  • 01:58Slides Preparation
  • 02:31Immunofluorescence
  • 04:07Analysis Through Fluorescence Microscope
  • 04:37Representative Results
  • 05:08Conclusions

Summary

Traduzione automatica

Traduzione automatica

This protocol presents a method to assess the formation and repair of DNA double-strand breaks through the simultaneous detection of γH2AX and 53BP1 foci in interphase nuclei of bleomycin-treated human peripheral lymphocytes.

Tags

Keywords: Dual ImmunofluorescenceγH2AX53BP1Human Peripheral LymphocytesDNA DamageDouble-strand BreaksBleomycinCell CultureFixationAntibody StainingFluorescence Microscopy

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