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人外周淋巴细胞中γH2AX和53BP1的双重免疫荧光

JoVE Journal
Genetica
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JoVE Journal Genetica

Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

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人外周淋巴细胞中γH2AX和53BP1的双重免疫荧光

DOI:

10.3791/65472-v

•

05:34 min

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July 14, 2023

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Aurora Falaschi, Anna Chiaramonte², Serena Testi, Roberto Scarpato

¹Unità di Genetica, Dipartimento di Biologia,University of Pisa, ²Department of Women-Child-Newborn Obstetrics and Gynaecology,Foundation IRCCS Ca’ Granda Ospedale Maggiore Policlinico

Capitoli

00:15Introduction
00:45Preparation of Samples and Mutagenic Treatment
01:19Fixation
01:58Slides Preparation
02:31Immunofluorescence
04:07Analysis Through Fluorescence Microscope
04:37Representative Results
05:08Conclusions

Summary

Traduzione automatica

该协议提出了一种通过同时检测博来霉素处理的人外周淋巴细胞的间期核中的γH 2AX和53BP1病灶来评估DNA双链断裂的形成和修复的方法。

Tags

Keywords: Dual Immunofluorescence γH2AX 53BP1 Human Peripheral Lymphocytes DNA Damage Double-strand Breaks Bleomycin Cell Culture Fixation Antibody Staining Fluorescence Microscopy

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