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Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

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توليد خطوط خلايا خروج المغلوب المرتبطة بالسنترومير Protein-E CENP-E^-/- باستخدام نظام كريسبر / كاس 9

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Biologia
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JoVE Journal Biologia

Generation of Centromere-Associated Protein-E CENP-E^-/- Knockout Cell Lines using the CRISPR/Cas9 System

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توليد خطوط خلايا خروج المغلوب المرتبطة بالسنترومير Protein-E CENP-E^-/- باستخدام نظام كريسبر / كاس 9

Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

DOI:

10.3791/65476-v

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02:19 min

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June 23, 2023

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Meng-Fei Xu², Jie Chen², Yue Xu², Jing-Lian Zhang², Yi Zhou², Jie-Jie He², Shan Wu², Ya-Lan Wei⁴, Zhen-Yu She²

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁴Medical Research Center,Fujian Maternity and Child Health Hospital

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CENP-E CRISPR/Cas9 Knockout HeLa Cells Chromosome Alignment Mitotic Defects Cell Cycle Arrest Cell Death Kinetochore-microtubule Capture Spindle Assembly Checkpoint

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توليد خطوط خلايا خروج المغلوب المرتبطة بالسنترومير Protein-E CENP-E^-/- باستخدام نظام كريسبر / كاس 9

Transfection, Isolation, and Screening of the CENP-E Knockout HeLa Cells

Immunofluorescence Staining and High-Resolution Confocal Microscopy of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

Chromosome Preparation and Karyotype Analysis of CRISPR/Cas9-Mediated CENP-E Knockout HeLa Cells

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