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Two-Step Cell Digestion of Mouse Carotid Artery

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从小鼠颈动脉制备用于单细胞测序的单细胞悬液

JoVE Journal
Biologia

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JoVE Journal Biologia

Preparation of a Single-Cell Suspension from Mouse Carotid Arteries for Single-Cell Sequencing

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从小鼠颈动脉制备用于单细胞测序的单细胞悬液

Two-Step Cell Digestion of Mouse Carotid Artery

DOI:

10.3791/65863-v

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01:16 min

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January 19, 2024

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Fengchan Li, Zhen Zhu, Yun Du, Li Zhu^2,3, Chaojun Tang^2,3

¹Cyrus Tang Hematology Center, Cyrus Tang Medical Institute,Soochow University, ²Collaborative Innovation Center of Hematology of Jiangsu Province,Soochow University, ³Suzhou Key Laboratory of Thrombosis and Vascular Biology,Soochow University

Tags

Single-cell Suspension Mouse Carotid Arteries Single-cell Sequencing Carotid Stenosis Carotid Atherosclerosis Two-step Digestion Method Collagenase/DNase Trypsin Cell Viability Cell Concentration Vascular Smooth Muscle Cells (VSMCs)Fibroblasts Endothelial Cells (ECs)Macrophages And Dendritic Cells (M&DCs)

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从小鼠颈动脉制备用于单细胞测序的单细胞悬液

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