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Biologia dello sviluppo

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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

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从斑马鱼幼虫中分离肠道进行单细胞RNA测序

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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo

Gut Isolation from Zebrafish Larvae for Single-cell RNA Sequencing

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从斑马鱼幼虫中分离肠道进行单细胞RNA测序

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:

10.3791/65876-v

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01:24 min

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November 10, 2023

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Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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Keywords: Zebrafish Gut Isolation Single-cell RNA Sequencing Gastrointestinal Development Enteric Nervous System Intestinal Cell Types Epithelial Cells Stromal Cells Blood Cells Muscle Cells Immune Cells Enteric Neurons Enteric Glia ScRNA-seq Congenital Defects Enteric Neuromuscular Disorders Gastrointestinal Health And Disease

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