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Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

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Hurtig og effektiv berigelse af musens rygmarvsmikroglia

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia

Hurtig og effektiv berigelse af musens rygmarvsmikroglia

Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

DOI:

10.3791/65961-v

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01:52 min

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September 22, 2023

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Claudia I Gutiérrez-Román², María E Meléndez Camargo, Cuauhtémoc C García Rojas, Miguel Jimenez Olvera, Sandra H Gutiérrez Román, Oscar Medina-Contreras

¹Epidemiology, Endocrinology & Nutrition,Mexico Children’s Hospital, ²Pharmacy Department, National School of Biological Sciences,National Polytechnic Institute, ³Graduate Department,Dr. Eduardo Liceaga General Hospital of Mexico, ⁴Pain Clinic and Palliative Care,Dr. Eduardo Liceaga General Hospital of Mexico, ⁵General Surgery Service,Metropolitan Angeles Hospital

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Keywords: Spinal Cord Microglia Purification Isolation Flow Cytometry CD11b CD45 Collagenase Density Gradient Heterogeneity Central Nervous System Neurological Pathologies Chronic Pain

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