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Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord
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Arricchimento rapido ed efficiente della microglia del midollo spinale del topo
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Neuroscienze
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JoVE Journal Neuroscienze
Rapid and Efficient Enrichment of Mouse Spinal Cord Microglia
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Arricchimento rapido ed efficiente della microglia del midollo spinale del topo

Extraction and Enzymatic Purification of Microglia from the Mouse Spinal Cord

DOI:
10.3791/65961-v

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01:52 min

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September 22, 2023

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, , , , ,
1Epidemiology, Endocrinology & Nutrition,Mexico Children’s Hospital, 2Pharmacy Department, National School of Biological Sciences,National Polytechnic Institute, 3Graduate Department,Dr. Eduardo Liceaga General Hospital of Mexico, 4Pain Clinic and Palliative Care,Dr. Eduardo Liceaga General Hospital of Mexico, 5General Surgery Service,Metropolitan Angeles Hospital

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Keywords: Spinal CordMicrogliaPurificationIsolationFlow CytometryCD11bCD45CollagenaseDensity GradientHeterogeneityCentral Nervous SystemNeurological PathologiesChronic Pain

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