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Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm
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Imagerie en temps réel de la dynamique acrosomique du calcium et de l’exocytose dans les spermatozoïdes de souris vivants
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Real-Time Imaging of Acrosomal Calcium Dynamics and Exocytosis in Live Mouse Sperm
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Imagerie en temps réel de la dynamique acrosomique du calcium et de l’exocytose dans les spermatozoïdes de souris vivants

Combining Imaging with a Stimulant Delivery System to Study Live Mouse Sperm

DOI:
10.3791/65962-v

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00:46 min

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October 13, 2023

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, , , , ,
1Baker Institute for Animal Health, College of Veterinary Medicine,Cornell University, 2Department of Public and Ecosystem Health, College of Veterinary Medicine,Cornell University

Tags

Real-time ImagingAcrosomal Calcium DynamicsAcrosome ExocytosisSpermCalcium SignalingCapacitationFertilizationAcroSensEGenetically Encoded Calcium IndicatorGCaMPMCherryHigh Frame-rate ImagingStimulant Delivery SystemGenetic ToolsCRISPR

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Imagerie en temps réel de la dynamique acrosomique du calcium et de l’exocytose dans les spermatozoïdes de souris vivants
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