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Fabrication of the Thermoplastic Microfluidic Channels
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Biologia
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JoVE Journal Biologia
Fabrication of the Thermoplastic Microfluidic Channels

Fabrication of the Thermoplastic Microfluidic Channels

DOI:
10.3791/664-v

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16:00 min

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February 03, 2008

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, ,
1Department of Biomedical Engineering,Boston University

Capitoli

  • 00:05Introduction
  • 01:25Fabrication of the thermoplastic microfluidic channels
  • 04:51Fabrication of the micro-channels for porous polymer monolith
  • 10:11Preparation of bacterial sample
  • 13:12Isolation of DNA
  • 15:06Discussion

Summary

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Here we demonstrate how to fabricate thermoplastic microfluidic chips using hot embossing and heat sealing. Then we demonstrate how to use in situ light directed surface grafting and polymerization through the sealed chip to form the composite solid phase columns.

Tags

FabricationThermoplastic Microfluidic ChannelsNucleic AcidsPolymer/nanoparticle CompositeMicroscale LysisSolid Phase Extraction ColumnsPCRable SamplesHot EmbossingHeat SealingIn Situ Light Directed Surface GraftingPolymerizationComposite Solid Phase ColumnsCarbon Nanotube/polymer Composite ColumnBacterial Cell LysisSilica/polymer Composite ColumnProtocols

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