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Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

DOI:
10.3791/66527-v

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10:52 min

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March 22, 2024

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, , , ,
1Department of Bioengineering, Department of Medicine,University of Washington, 2Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Capitoli

  • 00:03Introduction
  • 01:16Passaging iPSCs
  • 03:32Embryoid Body Induction
  • 06:10Hematopoietic Differentiation
  • 08:31M-CSF Maturation and Osteoclast Differentiation
  • 09:25Representative Results
  • 10:19Conclusion

Summary

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This protocol presents the differentiation of human osteoclasts from induced pluripotent stem cells (iPSCs) and describes methods for the characterization of osteoclasts and osteoclast precursors.

Tags

OsteoclastsHuman Induced Pluripotent Stem Cells (iPSCs)DifferentiationCharacterizationPassagingCD34+ Peripheral Blood Mononuclear CellsBone Disease ResearchRicerca sul cancroEndoprosthesis ResearchExpandabilityDMEM/F-12DispaseHEPESCell CultureCell Aggregates

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