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破骨细胞与人诱导多能干细胞的分化和表征

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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo

Differentiation and Characterization of Osteoclasts from Human Induced Pluripotent Stem Cells

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破骨细胞与人诱导多能干细胞的分化和表征

DOI:

10.3791/66527-v

•

10:52 min

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March 22, 2024

•

Alexander Blümke², Jessica Simon, Elizabeth Leber, Marta Scatena, Cecilia M. Giachelli

¹Department of Bioengineering, Department of Medicine,University of Washington, ²Department of Orthopedics and Trauma Surgery, Medical Faculty Mannheim,Heidelberg University

Capitoli

00:03Introduction
01:16Passaging iPSCs
03:32Embryoid Body Induction
06:10Hematopoietic Differentiation
08:31M-CSF Maturation and Osteoclast Differentiation
09:25Representative Results
10:19Conclusion

Summary

Traduzione automatica

该方案介绍了人破骨细胞与诱导多能干细胞（iPSCs）的分化，并描述了破骨细胞和破骨细胞前体的表征方法。

Tags

Keywords: Osteoclasts Human Induced Pluripotent Stem Cells (iPSCs)Differentiation Characterization Passaging CD34+ Peripheral Blood Mononuclear Cells Bone Disease Research Ricerca sul cancro Endoprosthesis Research Expandability DMEM/F-12 Dispase HEPES Cell Culture Cell Aggregates

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