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Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

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Isolering af adeno-associerede virale vektorer gennem en enkelttrins og halvautomatisk heparinaffinitetskromatografiprotokol

JoVE Journal
Bioingegneria

This content is Free Access.

JoVE Journal Bioingegneria

Isolation of Adeno-Associated Viral Vectors Through a Single-Step and Semi-Automated Heparin Affinity Chromatography Protocol

Isolering af adeno-associerede virale vektorer gennem en enkelttrins og halvautomatisk heparinaffinitetskromatografiprotokol

Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

DOI:

10.3791/66550-v

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01:57 min

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April 05, 2024

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Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^2,4, Carina Henriques^2,5, Ana C. Silva^2,3, Diana D. Lobo^2,3, Luísa Cortes^2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC – Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB – Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC – Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector – Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC – Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC – Microscopy Imaging Center of Coimbra – CNC,University of Coimbra

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Keywords: Adeno-associated Viral Vectors AAV Purification Heparin Affinity Chromatography Transient Transfection Density Gradient Ultracentrifugation Scalable Production Semi-automated Protocol Mosaic AAV Preclinical Studies

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