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Capitoli
- 00:01Titolo
- 00:55Introduction
- 01:41Labeling of Human Mesenchymal Stem Cells with Fluorescent Dye DiD
- 03:12Labeling of Human Embryonic Stem Cells with the Fluorescent Dye ICG
- 06:23Imaging Stem Cells in vivo
- 06:59Conclusion
This video shows techniques for labeling of human embryonic stem cells and mesenchymal stem cells with fluorescent dyes. This technique can be used for an in vivo tracking of transplanted stem cells with optical imaging and for histopathological correlations with fluorescence microscopy.
Tags
LabelingStem CellsFluorescent DyesNon-invasive DetectionOptical ImagingIn Vivo MonitoringEx Vivo LabelingIntravenous InjectionVisualizationTarget OrgansPathologiesHuman Mesenchymal Stem Cells (hMSC)Lipophilic Fluorescent Dye DiDHuman Embryonic Stem Cells (hESC)FDA Approved Fluorescent Dye Indocyanine Green (ICG)Uptake MechanismAdherenceDiffusionPhospholipid Cell Membrane BilayerLabeling EfficiencySerum-free MediaSerum-containing MediaTransfection Agent Protamine SulfateContrast Agent Uptake
