È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:00Titolo
- 00:14Introduction
- 01:11Micrococcal Nuclease Treatment
- 02:27Hypotonisation
- 07:09Elution
- 08:00Protein Digestion and Pheno Chloroform Extraction
- 10:44Conclusion
Until recently, expression studies on human brain were limited to quantification of RNA or protein. With the chromatin immunoprecipitation techniques described in this paper, it will be possible to map histone methylation and other epigenetic regulators of gene expression in postmortem brain.
Tags
Chromatin AssayNeuropsychiatric IllnessesSchizophreniaBipolar DiseaseAutismEpigenetic AlterationsGene ExpressionMolecular PathologyPostmortem Brain ResearchMRNA QuantificationProtein QuantificationAutolysis TimeChromatin StructuresNucleosomal OrganizationDNA:core Histone BindingMethylation PatternCovalent ModificationsCore HistonesGenomic LociNative Chromatin Immunoprecipitation (NChIP)Frozen Brain SpecimensMicrococcal Nuclease DigestionQPCREpigenetic Mechanisms
