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一个人脑组织中的染色质含量
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JoVE Journal Biologia
A Chromatin Assay for Human Brain Tissue
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一个人脑组织中的染色质含量

DOI:
10.3791/717-v

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11:31 min

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March 21, 2008

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1Psychiatry, Brudnick Neuropsychiatric Research Institute,University of Massachusetts Medical School

Capitoli

  • 00:00Titolo
  • 00:14Introduction
  • 01:11Micrococcal Nuclease Treatment
  • 02:27Hypotonisation
  • 07:09Elution
  • 08:00Protein Digestion and Pheno Chloroform Extraction
  • 10:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

直到最近，表达对人类大脑的研究仅限于RNA或蛋白质的定量。本文中所描述的染色质免疫沉淀技术，将有可能映射在死后大脑基因表达的组蛋白甲基化和其他后生监管。

Tags

Chromatin AssayNeuropsychiatric IllnessesSchizophreniaBipolar DiseaseAutismEpigenetic AlterationsGene ExpressionMolecular PathologyPostmortem Brain ResearchMRNA QuantificationProtein QuantificationAutolysis TimeChromatin StructuresNucleosomal OrganizationDNA:core Histone BindingMethylation PatternCovalent ModificationsCore HistonesGenomic LociNative Chromatin Immunoprecipitation (NChIP)Frozen Brain SpecimensMicrococcal Nuclease DigestionQPCREpigenetic Mechanisms

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