/

/

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

JoVE Journal
Biologia

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Biologia

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

From MEFs to Matrigel I: Passaging hESCs in the Presence of MEFs

DOI:

•

06:29 min

•

June 04, 2008

•

Jin Zhang, Ivan Khvorostov, Michael Teitell

¹David Geffen School of Medicine,University of California, Los Angeles

Capitoli

00:00From MEFs to Matrigel
00:38Introduction
01:30Splitting hESCs plated on mouse embryonic fibroblasts (MEFs)
06:12Conclusion

Summary

Traduzione automatica

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells. Part 1 of 3.

Tags

MEFs Matrigel Passaging HESCs Grow HESCs Mouse Embryonic Fibroblast Feeder Cells Embryonic Stem Cells

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Passaging Human Neural Stem Cells

Labeling hESCs and hMSCs with Iron Oxide Nanoparticles for Non-Invasive in vivo Tracking with MR Imaging

Generating iPS Cells from MEFS through Forced Expression of Sox-2, Oct-4, c-Myc, and Klf4

From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells

Generation of Myospheres From hESCs by Epigenetic Reprogramming

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

High-resolution Respirometry to Measure Mitochondrial Function of Intact Beta Cells in the Presence of Natural Compounds

Measuring Crop Motility and Food Passaging in Drosophila

Read Article