È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:03hESCs and Mitochondrial Complex Activity
- 00:44Introduction
- 01:41Mitochondrial Isolation
- 04:08Solubilizing the Crude Mitochondrial Pellet
- 05:42Gel and Sample Preparation and Electrophoresis Conditions
- 09:47In-Gel Activity Assays
- 12:08Conclusion
Tags
Mitochondrial Complex ActivityHuman Embryonic Stem CellsCellular MetabolismHomeostasisCell Signaling NetworkProgrammed Cell DeathATP ProductionCytoplasmic Redox StateCa2+ BalanceFatty Acid CatabolismHeme SynthesisNucleotide SynthesisSteroid Hormone SynthesisAmino Acid SynthesisIron-sulfur Cluster AssemblyHeat ProductionMitochondrial Genome MutationsAging CorrelationCancer Development CorrelationStem Cell ViabilityStem Cell ProliferationStem Cell Differentiation CapacityEnergy Status Of Stem CellsMitochondrial Properties Of Human Embryonic Stem Cells (hESCs)Precursor Progeny DifferentiationMeasurement Of Mitochondrial Respiratory Complexes
