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Counting and Determining the Viability of Cultured Cells
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Biologia
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JoVE Journal Biologia
Counting and Determining the Viability of Cultured Cells

Counting and Determining the Viability of Cultured Cells

DOI:
10.3791/752-v

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05:47 min

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June 23, 2008

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,
1Molecular Pathology Laboratory Network, Inc

Capitoli

  • 00:00Titolo
  • 00:36Introduction
  • 00:57Preparing the hemacytometer
  • 01:31Preparing cell suspension
  • 02:08Loading hemacytometer
  • 02:42Counting and calculating number of cells
  • 04:24Determination of cell viability
  • 05:25Conclusion

Summary

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Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.

Tags

CountingDeterminingViabilityCultured CellsStandardizationCulture ConditionsQuantitation ExperimentsHemacytometerGlass SlideCounting ChamberCell SuspensionCell Density Calculation

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