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Primary Culture of Hippocampal Neurons from P0 Newborn Rats
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Biologia
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JoVE Journal Biologia
Primary Culture of Hippocampal Neurons from P0 Newborn Rats

Primary Culture of Hippocampal Neurons from P0 Newborn Rats

DOI:
10.3791/895-v

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07:18 min

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September 29, 2008

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1Department of Psychology,Michigan State University (MSU)

Capitoli

  • 00:00Titolo
  • 00:15Introduction
  • 01:04Preparing Tools and Reagents
  • 02:40Isolating the Hippocampi
  • 04:21Dissociation of Hippocampi
  • 06:09Imaging Hippocampal Neurons
  • 07:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

The dissection and growth of cells from an individual brain area facilitates investigation of cellular and physiological parameters. We describe a method for primary cell culturing that produces neuron-enriched cultures in a serum-free environment.

Tags

Hippocampal NeuronsP0 Newborn RatsPrimary CulturePhysiological PropertiesInvolvementLearning And MemoryNeuroscientistsIndividual CellSingle Synapse LevelIsolateGrowCulturesTwo WeeksRatiometric Calcium ImagingProtocolBrain Regions

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