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Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH
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Biologia
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JoVE Journal Biologia
Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH

Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH

DOI:
10.3791/907-v

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09:59 min

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August 14, 2008

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, , , ,
1Department of Physiology and Biophysics,University of California, Irvine (UCI), 2Department of Neurobiology and Behavior,University of California, Irvine (UCI)

Capitoli

  • 00:01Titolo
  • 00:07Introduction
  • 00:38Induction of Adoptive DTH and Measuring Inflammation in the Ear
  • 02:54Removing Dermal Layers from Ear
  • 05:27Preparing Ear for 2-photon Imaging
  • 07:48Imaging the T cell Motility in the Ear
  • 09:26Conclusion

Summary

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Traduzione automatica

Here we demonstrate a method for inducing and recording the progress of a delayed type-hypersensitivity (DTH) reaction in the rat ear. This is followed by a demonstration of the preparation of rat ear tissue for two-photon imaging of the effector / memory T cell response.

Tags

ImagingEffector Memory T CellsEarInductionAdoptive DTHDelayed Type HypersensitivityImmune ReactionCCR7-T LymphocytesMethodRat EarTwo-photon ImagingGFP-labeledOva-specificCell LineEquilibrateChallengeSalineOva Conjugated To Texas-RedAntigen-presenting CellsMotility Of CellsDeep Dermal LayerImmune ResponseCollagen FibersSecond Harmonic Generation

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