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In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts
JoVE Journal
Biologia
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JoVE Journal Biologia
In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

DOI:
10.3791/908-v

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04:49 min

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September 02, 2008

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1Department of Cell Biology,Emory University

Capitoli

  • 00:00Titolo
  • 00:50Introduction
  • 01:22Performing the Nuclear Assembly Reaction
  • 02:54Assessing Nuclei Formation
  • 04:18Conclusion

Summary

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Traduzione automatica

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

Tags

In Vitro Nuclear AssemblyFractionated Xenopus Egg ExtractsNuclear Membrane AssemblyCell Division CycleXenopus Sperm ChromatinCytosolLight Membrane FractionsReconstituted NucleiNormal Nuclear Processes

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