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1Department of Cancer Genetics and Developmental Biology,BC Cancer Research Centre, 2Interdisciplinary Oncology Program,University of British Columbia – UBC, 3Department of Pathology and Laboratory Medicine,University of British Columbia – UBC, 4Photography/Video Production, Multi-Media Services,BC Cancer Agency, 5Department of Medical Genetics, Life Sciences Institute,,University of British Columbia – UBC
Capitoli
- 00:06Introduction
- 02:06The MeDIP Protocol
- 02:25Materials
- 02:56DNA Preparation
- 04:52DNA Sonication
- 06:33Immunoprecipitation of Methylated DNA
- 13:51DNA Purification
- 19:27PCR Validation
- 21:09Credits
Questo video dimostra il protocollo di immunoprecipitazione DNA metilato (MeDIP). MeDIP è una procedura di due giorni che estrae selettivamente i frammenti di DNA metilato da un campione di DNA genomico utilizzando anticorpi con specificità per 5-metilcitosina (anti-5 MC).
Tags
Methylated DNA ImmunoprecipitationDNA Methylation PatternsEpigeneticsGene ExpressionNormal DevelopmentDiseaseMethylation ProfilesMeDIPMethylated DNA ExtractionAntibody ReactionImmunoprecipitated DNAInput DNAHeat DenaturationAnti-5’mC AntibodyMagnetic BeadsSecondary AntibodyAntibody ComplexSeparation Of Methylated DNANon-methylated DNA Removal
