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补丁钳爪蟾卵母细胞中表达的离子通道
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Biologia
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JoVE Journal Biologia
Patch Clamp Recording of Ion Channels Expressed in Xenopus Oocytes
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补丁钳爪蟾卵母细胞中表达的离子通道

DOI:
10.3791/936-v

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08:00 min

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October 16, 2008

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, ,
1Department of Molecular and Cellular Physiology,Stanford University , 2Department of Molecular and Cellular Physiology,Stanford University School of Medicine

Capitoli

  • 00:00Titolo
  • 00:49Introduction
  • 01:35Removing the Vitelline Membrane
  • 03:33Gigaseal Formation
  • 06:05Outside-Out Topology
  • 06:59Conclusion

Summary

Traduzione automatica

Traduzione automatica

这是为了介绍，从非洲爪蟾卵母细胞来修补钳记录。它涵盖了卵黄膜去除，形成一个gigaohm密封（gigaseal），和可选的补丁外拓扑的转换。

Tags

Patch Clamp RecordingIon ChannelsXenopus OocytesElectrophysiological MeasurementHeterologous CellsAfrican Clawed FrogXenopus LaevisMacropatchSingle-channel RecordingOocyte PreparationSeal GenerationSuccessful Seal Generation

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