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Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Biologia
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JoVE Journal Biologia
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

DOI:
10.3791/939-v

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05:30 min

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October 08, 2008

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, ,
1Department of Molecular and Cellular Physiology,Stanford University , 2Department of Molecular and Cellular Physiology,Stanford University School of Medicine

Capitoli

  • 00:02Titolo
  • 00:33Introduction
  • 01:04Pulling Pipettes
  • 03:28Fire Polishing Pipettes
  • 04:42Conclusion

Summary

Traduzione automatica

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This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.

Tags

Patch-pipettesSharp ElectrodesGlass MicroelectrodesProgrammable PullerElectrophysiologyCapillary GlassMetal FilamentLaserGravityMotorPatch-clamp RecordingIntracellular RecordingHeatCRNA InjectionXenopus OocytesDiameterOuter Diameter

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