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Capitoli
- 00:00Titolo
- 00:29Preparing the lipids
- 02:13Determining how much ICAM-1 and MHC are deposited on the planar bilayer
- 03:22Cleaning the glass cover slips for forming planar bilayers
- 04:47Forming the bilayers
- 08:27Charging the bilayers and introducing His-tagged proteins
- 10:20Introducing primary T cells into the flow cell
- 11:36Conclusion
Tags
Supported Planar BilayersImmunological SynapseKinapseMolecular InteractionsLymphocytesAntigen Presenting CellsNi2+-chelating LipidsRecombinant Cell Adhesion ProteinsMHC ProteinsPoly-histidine TagsGlass SurfacesSpecialized ChamberFlow-cellTIRF MicroscopySynapse FormationT Cell LocomotionReceptor SortingReceptor Degradation
