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产后小鼠小脑颗粒神经元祖细胞和神经元的分离和培养

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation and Culture of Post-Natal Mouse Cerebellar Granule Neuron Progenitor Cells and Neurons

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产后小鼠小脑颗粒神经元祖细胞和神经元的分离和培养

DOI:

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16:04 min

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January 16, 2009

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Hae Young Lee, Lloyd A. Greene, Carol A. Mason³, M. Chiara Manzini⁴

¹Department of Genetics and Development,Columbia University , ²Department of Pathology and Cell Biology,Columbia University , ³Department of Neuroscience,Columbia University , ⁴Department of Neurology, Beth Israel Deaconess Medical Center,Harvard Medical School

Capitoli

00:00Titolo
01:10Introduction
01:37Preparing for Cerebellar Dissection
03:15Cerebellar Dissection and Meninges Removal
06:01Cerebellar Dissection and Meninges Removal
09:29Percoll Gradient Separation
12:03Pre-plating and Plating
13:23Risultati
14:32Conclusion

Summary

Traduzione automatica

在这里，我们提出了一个方法来隔离和文化的小脑颗粒神经元祖细胞和产后小鼠小脑颗粒神经元。

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