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A Protocol for the Production of KLRG1 Tetramer

JoVE Journal
Biology

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JoVE Journal Biology

A Protocol for the Production of KLRG1 Tetramer

A Protocol for the Production of KLRG1 Tetramer

DOI:

•

07:24 min

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January 12, 2010

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Stephanie C. Terrizzi, Cindy Banh, Laurent Brossay

¹Department of Molecular Microbiology and Immunology,Brown University

Chapters

00:00Title
00:08Introduction
00:33Preparation of Inclusion Bodies
03:07Refolding of KLRG1
04:41Concentration and Purification of Refolded KLRG1 Tetramers
06:25Size Exclusion Chromatography of Refolded KLRG1
06:58Conclusion

Summary

Automatic Translation

This protocol describes the production of KLRG1 tetramer, which is a powerful tool for the analysis of KLRG1 ligands.

Tags

KLRG1 Tetramer Production Protocol Killer Cell Lectin-like Receptor G1 C Type Lectin-like Superfamily Monomer Disulfide-linked Homodimer NK Cells Effector/memory T Cells KLRG1 Ligands E-cadherin N-cadherin R-cadherin Ca2+-dependent Cell-cell Adhesion Molecules Cadherin Repeats Transmembrane Domain Cytoplasmic Domain Actin Cytoskeleton Immune Response

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