JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
DOI:
10.3791/51408-v
•
09:03 min
•
May 29, 2014
•
HoTae Lim
,
In Young Choi
,
Gabsang Lee
1
Institute for Cell Engineering, Department of Neurology and Neuroscience
,
Johns Hopkins University School of Medicine
Chapters
00:05
Title
01:20
Preparing hESCs for FACS Purification
02:26
Lysis of FACS-purified Single Cell in Each Well of a 96-well Plate
03:28
Reverse Transcription
04:29
Amplification
05:26
qRT-PCR Performance
06:21
Results: qRT-PCR is an Efficient Method for Profiling Single Cell Gene Expression
08:39
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
Single cell gene expression assay is needed for understanding stem cell heterogeneities.
Tags
Single Cell
Transcriptome
QRT-PCR
Heterogeneity
Stem Cell
Differentiation
OCT4
NANOG
Gene Expression
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
check_url/51408?article_type=v
Propagation of Human Embryonic Stem (ES) Cells
check_url/51408?article_type=v
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
check_url/51408?article_type=v
Derivation of Human Embryonic Stem Cells by Immunosurgery
check_url/51408?article_type=v
Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
check_url/51408?article_type=v
Chromatin Immunoprecipitation from Human Embryonic Stem Cells
check_url/51408?article_type=v
In vitro Labeling of Human Embryonic Stem Cells for Magnetic Resonance Imaging
check_url/51408?article_type=v
Culture and Maintenance of Human Embryonic Stem Cells
check_url/51408?article_type=v
Chromosomal Spread Preparation of Human Embryonic Stem Cells for Karyotyping
check_url/51408?article_type=v
Freezing and Thawing Human Embryonic Stem Cells
check_url/51408?article_type=v
Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
Read Article