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Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines
JoVE Journal
Genetics
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JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

Chromatin Immunoprecipitation (ChIP) in Mouse T-cell Lines

DOI:
10.3791/55907-v

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11:39 min

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June 17, 2017

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, ,
1Institute of Biochemistry,University of Giessen

Chapters

  • 00:05Title
  • 01:01Crosslinking the Cells, Cell Lysis, and Chromatin Shearing
  • 03:31Determination of Shearing Efficiency
  • 05:55Immunoprecipitation
  • 08:09DNA Purification
  • 09:09Results: Shearing Efficiency and Distribution of Chromatin Marks in Mouse T-cell Lines
  • 10:09Conclusion

Summary

Automatic Translation

Automatic Translation

This work describes a protocol for chromatin immunoprecipitation (ChIP) using a mature mouse T-cell line. This protocol is suitable to investigate the distribution of specific histone marks at specific promoter sites or genome-wide.

Tags

Chromatin ImmunoprecipitationChIPMouse T-cell LinesHistone MarksChromatin BiologyChromatin ShearingFormaldehyde CrosslinkingGlycine QuenchingSonicationFocused UltrasonicatorLysis BufferCell PelletSupernatant

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