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A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying
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A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying

A 3-dimensional (3D)-printed Template for High Throughput Zebrafish Embryo Arraying

DOI:
10.3791/57892-v

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04:52 min

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June 01, 2018

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, ,
1College of Environmental Science and Engineering, State Key Laboratory of Pollution Control and Resource Reuse,Tongji University, 2UNEP-Tongji Institute of Environment for Sustainable Development,Tongji University

Chapters

  • 00:05Title
  • 00:52Designing of an Arraying Template and Zebrafish Embryo Spawning
  • 02:18Arraying Zebrafish Embryos into a 96-well Plate
  • 03:48Results: Representative Microscopic Images of Embryos After 6-well Plate Transferrence
  • 04:24Conclusion

Summary

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Here, we present a protocol to design and fabricate a zebrafish embryo arraying template, followed by a detailed procedure on the use of such template for high throughput zebrafish embryo arraying into a 96-well plate.

Tags

3D-printed TemplateHigh ThroughputZebrafish EmbryoArrayingAutomationZebrafish Screening96-well Plate384-well PlateEmbryo EntrapmentVacuum-based ArrayingTemplate Design3D PrintingZebrafish SpawningEmbryo CollectionEmbryo Selection

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