Journal
/
Immunology and Infection
/
A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses

A Galleria mellonella Oral Administration Model to Study Commensal-Induced Innate Immune Responses

DOI:
10.3791/59270-v

•

06:32 min

•

March 21, 2019

•

, ,
1Department for Medical Microbiology, Hygiene, Interfacultary Institute for Microbiology, Infection Medicine,University of Tübingen

Chapters

  • 00:04Title
  • 00:28G. mellonella Rearing and Larva Preparation and Force Feeding
  • 01:53Orally-Administered Larva Processing and RNA Isolation
  • 04:22Results: Representative Commensal-Induced Gene and Protein Expression in G. mellonella
  • 05:33Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we provide a detailed protocol for an oral administration model using Galleria mellonella larvae and how to characterize induced innate immune responses. Using this protocol, researchers without practical experience will be able to use the G. mellonella force-feeding method.

Tags

Galleria MellonellaOral AdministrationInnate Immune ResponsesCommensal BacteriaInfection ModelPathogenic BacteriaLarvaeSubstrateForce FeedingHomogenizationRNA ExtractionTRIzol1-Bromo-3-chloropropane

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59270?article_type=v

The Insect Galleria mellonella as a Powerful Infection Model to Investigate Bacterial Pathogenesis
check_url/59270?article_type=v

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
check_url/59270?article_type=v

Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
check_url/59270?article_type=v

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production
check_url/59270?article_type=v

Using RNA-interference to Investigate the Innate Immune Response in Mouse Macrophages
check_url/59270?article_type=v

Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection
check_url/59270?article_type=v

The Galleria mellonella Waxworm Infection Model for Disseminated Candidiasis
check_url/59270?article_type=v

A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection
check_url/59270?article_type=v

Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex
check_url/59270?article_type=v

Isolation of Tonsillar Mononuclear Cells to Study Ex Vivo Innate Immune Responses in a Human Mucosal Lymphoid Tissue

Read Article