Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture

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Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE

Published: May 19, 2023

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10.3791/200258

Lisa A. Krattiger, Maria Mitsi, Benjamin R. Simona, Martin Ehrbar

¹Department of Obstetrics,University Hospital Zurich, University of Zurich, ²Ectica Technologies AG

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Keywords: 3D Stromal-endothelial Cell Co-culture GFP Signal Fluorescence Microscopy Image Pre-processing Fiji/ImageJ Brightness/contrast Adjustment Median Blur Filter Image Binning Angiogenesis Analyzer Network Quantification Total Network Length Number Of Junctions Isolated Segments FGF-2 Growth Factors

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Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture. J. Vis. Exp. (Pending Publication), e200258, doi:10.3791/200258 (2023).

Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE

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