Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs) - JoVE

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Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs)

Published: June 30, 2023

doi:

Jiale Cheng², Jiong Zhu², Yujiao Liu³, Chen Yang³, Yuehui Zhang, Yuchen Liu, Chaozhi Jin, Jian Wang²

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

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Keywords: Peptide Extraction Small Extracellular Vesicles SEVs Ultracentrifugation Protease Inhibitor Ultrasonic Crushing Dithiothreitol Iodoacetamide Ultrafiltration Desalting Trifluoroacetic Acid Acetonitrile Mass Spectrometry

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Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs). J. Vis. Exp. (Pending Publication), e200348, doi:10.3791/200348 (2023).

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