Agrobacterium-Mediated Transformation of <i>Chlorella vulgaris </i> and Confirmation of Gene Integration via Colony PCR (cPCR)

doi:10.3791/200638

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Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR) (Video) | JoVE

Published: October 27, 2023

doi:

10.3791/200638

M. Reza Roushan, Chuchi Chen, Parisa Ahmadi, Valerie C. A. Ward

¹Department of Chemical Engineering,University of Waterloo

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Agrobacterium-mediated Transformation Chlorella Vulgaris Colony PCR (cPCR)Gene Integration Log Phase Culture TAP Agar Supplemented LB Broth Agrobacterium Tumefaciens Induction Medium Selective Media Tetracycline DNA Agarose Gel

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Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (cPCR). J. Vis. Exp. (Pending Publication), e200638, doi:10.3791/200638 (2023).

Agrobacterium-Mediated Transformation of Chlorella vulgaris and Confirmation of Gene Integration via Colony PCR (CPCR) (Video) | JoVE

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