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Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API) (Video) | JoVE

Published: September 08, 2023

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Christophe Bontoux^2,3,4,5, Virginie Lespinet-Fabre^2,3,4, Olivier Bordone^2,3,4, Virginie Tanga^2,3,4, Maryline Allegra^2,3,4, Myriam Salah^2,3,4, Salomé Lalvée^2,3,4, Samantha Goffinet^2,3,4, Jonathan Benzaquen^4,5,6, Charles-Hugo Marquette^4,5,6, Marius Ilié^2,3,4,5, Véronique Hofman^2,3,4,5, Paul Hofman^2,3,4,5

¹Laboratory of Clinical and Experimental Pathology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur, CHU de Nice, ²Hospital-Integrated Biobank (BB-0033-00025),Université Côte d’Azur, Hôpital Pasteur, CHU de Nice, ³Institut Hospitalo-Universitaire (IHU), RespirERA,Université Côte d’Azur, Hôpital Pasteur, CHU de Nice, ⁴FHU OncoAge,Université Côte d’Azur, ⁵Team 4, Institute of Research on Cancer and Aging (IRCAN), CNRS INSERM, Centre Antoine-Lacassagne,Université Côte d’Azur, ⁶Department of Pulmonary Medicine and Thoracic Oncology, Centre Hospitalier Universitaire de Nice,Université Côte d’Azur

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Keywords: FFPE DNA RNA Sequencing Automated Purification Instrument API Integrated Genomic Viewer Protease Digest Centrifugation DNA Digestion Nucleic Acid Purification Plate

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Preparation of FFPE DNA and RNA Samples for Sequencing Using an Automated Purification Instrument (API). J. Vis. Exp. (Pending Publication), e200724, doi:10.3791/200724 (2023).

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