Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein

Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein (Video) | JoVE

Published: August 25, 2023

doi:

10.3791/200736

Sarah Wazir, Danny Farhat, Mahalashmi Srinivasan², Jyh-Yeuan Lee

¹Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, ²Department of Biochemistry, Faculty of Medicine and Dentistry,University of Alberta

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Keywords: Reductive Methylation ABCG5/G8 Protein Purification Pre-crystallization Treatment Nickel-NTA Column PD-10 Desalting Cholesterol Addition Ultracentrifugation Protein Concentration Analytical Gel Filtration

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Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein. J. Vis. Exp. (Pending Publication), e200736, doi:10.3791/200736 (2023).

Reductive Methylation and Pre-Crystallization Treatment of Purified Heterodimeric ABCG5/G8 Protein (Video) | JoVE

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