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Fluorometric Quantification and Cytometric Analysis of Rat Neutrophil Extracellular Traps (NETs)

Published: April 26, 2024

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Xiangfeng Gong*¹, Yutao Sun*¹, Xiaoxin Zhang³, Zhenghua Xiao⁴, Li He⁴, Chaoyi Qin⁴

¹Department of Cardiovascular Surgery, West China Hospital,Sichuan University, ²Department of Integrated Traditional Chinese and Western Medicine, Sichuan Provincial Pancreatitis Centre, West China Hospital,Sichuan University, ³West China-Liverpool Biomedical Research Centre, West China Hospital,Sichuan University, ⁴Cardiovascular Surgery Research Laboratory, West China Hospital,Sichuan University

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Keywords: Fluorometric Quantification Cytometric Analysis Rat Neutrophil Extracellular Traps (NETs)Tris-EDTA Buffer DNA Standards Assay Reagent Fluorescence Microplate Reader Nucleus Stain Cell-free DNA Stain Cytometer SYTOX Green HEXT Channel PMA Stimulation Cell-free DNA Content

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Fluorometric Quantification and Cytometric Analysis of Rat Neutrophil Extracellular Traps (NETs). J. Vis. Exp. (Pending Publication), e200812, doi:10.3791/200812 (2024).

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